Can short-term exposure to copper and atrazine be cytotoxic to microalgae?
2020
Guidony, Nicole Soares | Lopes, Fernanda Moreira | Guimarães, Pablo Santos | Escarrone, Ana Laura Venquiaruti | Souza, Marta Marques
Aquatic environments can be easily contaminated due to anthropogenic activities that may affect local biota. Microalgae are abundant and have an important role on the food chain. Consequently, they stand out as promising models for studies of contaminants. This study investigated the cytotoxic effects of atrazine and copper (separate and mixture) exposure in microalgae Desmodesmus communis, as well as its cellular defense due to ABC (ATP-binding cassette) proteins activity against the xenobiotics. We analyzed two different ABC proteins activity pathways: P-gp, which is responsible for nonspecific substance efflux, and MRP that is associated with metals efflux. It was observed that the microalgae exposure to atrazine (90 nM) and copper (141 nM) has been considered cytotoxic. When contaminants were mixed, only the combination of both highest concentrations tested was cytotoxic. The P-gp blocker, verapamil, demonstrated that the contaminants tested caused proteins inhibition. However, the MK-571 (MRP blocker) did not block pump activity. There was an inverse relationship between ABC protein activity and cytotoxicity; non-cytotoxic conditions suggest increased activity of microalgae defense proteins.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par National Agricultural Library
Découvrez la collection de ce fournisseur de données dans AGRIS
