Investigation of Organic Solvent Resistance Mechanisms in Vibrio alginolyticus IBBCₜ₂
2012
Stancu, Mihaela Marilena
Constanta harbor has been contaminated for decades with petroleum and petroleum products, which contain different toxic organic solvents. A novel solvent-tolerant bacterium, Vibrio alginolyticus IBBCₜ₂ was isolated from a seawater sample (Constanta harbor). Alkanes (i.e., n-hexane, n-decane, cyclohexane) with logarithm of partition coefficient in n-octanol and water (log P OW ) > 3.35, were less toxic for V. alginolyticus strain IBBCₜ₂, compared with aromatics (i.e., toluene, m-xylene, ethylbenzene) with log P OW < 3.17. The high organic solvent resistance of V. alginolyticus IBBCₜ₂ could be due to the presence of some catabolic (alkB, alkB/alkB1, todC1, xylM, C23DO) and transporter (HAE1, acrAB) genes. The adaptation mechanisms, underlying cyclohexane, n-hexane, n-decane, toluene, m-xylene, and ethylbenzene resistance in V. alginolyticus IBBCₜ₂ showed a complex response of cells 60 min after solvent shock (i.e., modifications of the cell viability, changes in the membrane’s lipid and protein profile, modifications of the genomic fingerprinting). Exposure of V. alginolyticus IBBCₜ₂ cells to salt stress decreases the organic solvents tolerance of this bacterium.
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