3-Phenyllactic acid production by substrate feeding and pH-control in fed-batch fermentation of Lactobacillus sp. SK007
2009
Mu, Wanmeng | Liu, Fengli | Jia, Jianghua | Chen, Chao | Zhang, Tao | Jiang, Bo
3-Phenyllactic acid (PLA), which is produced by some strains of lactic acid bacteria (LAB), is a known antimicrobial agent with a broad spectrum. Batch and fed-batch fermentation by the strain Lactobacillus sp. SK007 for PLA production have been reported. With batch fermentation without pH-control, PLA production yield was 2.42gL⁻¹. When fed-batch fermentation by Lactobacillus sp. SK007 was conducted in 3L initial volume with pH-control at 6.0 and intermittent feeding, which was developed after fermentation for 12h and every 2h with 120mL 100gL⁻¹ PPA phenylpyruvic acid (PPA) and 50mL 500gL⁻¹ glucose each time, PLA production yield reached 17.38gL⁻¹. The final conversion ratio of PPA to PLA was 51.1%, and the PLA production rate was 0.241gL⁻¹ h⁻¹. This indicated that PPA was the ideal substrate for PLA fermentation production, and fed-batch fermentation with intermittent PPA feeding and pH-control was an effective approach to improve PLA production yield.
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