Microspore embryogenesis and fertile plantlet regeneration in a salt susceptible X salt tolerant rice hybrid
2001
Chowdhury, B. | Mandal, A.B.
Shed microspore embryogenesis and fertile plantlet regeneration were observed in a salt susceptible x salt tolerant indica rice F1 hybrid involving IR 24 and CRM 30. The in vitro culture response and regeneration of green plantlets in the hybrid were superior to those of the parents. Direct embryogenesis and plantlet regeneration with multiple tillers were observed in shed microspore embryos. In intact anther culture, plantlet development from microspore involved a callus phase. The number of multiple tillers developed through secondary embryogenesis was almost equal in both the cases. However, the results indicate that regeneration of green plantlets was higher in case of shed microspore culture in liquid medium containing the synthetic polymer Ficoll 400 than from intact anthers cultured on a semi-solid system. Shed microspore culture produced a number of double haploids, which may result in far reaching consequences in genetic improvement of rice.
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