Insecticidal and synergistic activity of dsRNAs targeting buprofezin‐specific genes against the small brown planthopper, Laodelphax striatellus
2020
Fang, Ying | Park, Min G. | Choi, Jae Y. | Park, Dong H. | Wang, Minghui | Kim, Hyun J. | Kim, Woo J. | Je, Yeon H.
The small brown planthopper, Laodelphax striatellus, is a dangerous pest in rice fields. Although buprofezin has been used to control L. striatellus for more than a decade, the occurrence of buprofezin‐resistant L. striatellus has been recently reported. To develop an alternative pest control strategy, comparative transcriptome analysis of buprofezin‐treated and nontreated L. striatellus was performed to screen the buprofezin‐specific target genes for RNA interference (RNAi) application. Among six genes downregulated in the buprofezin‐treated L. striatellus, RNAi‐based silencing of the lipophorin precursor, endocuticle structure glycoprotein, and chitin synthase significantly induced the lethality of L. striatellus in a concentration‐dependent manner. In addition, a cocktail of double‐stranded RNAs against these three genes showed synergistic effects with buprofezin. These results provide RNAi‐based effective approaches to control L. striatellus as well as an efficient method to identify novel target genes for RNAi application.
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