Quantitative analysis of triterpenes friedelan-3-beta-ol and friedelin in Maytenus aquifolium by high-resolution gas chromatography and high-temperature capillary gas chromatography
2000
Nossack, A.C. | Vasconcelos, E.C. de | Vilegas, J.H.Y. | Lancas, F.M. | Roque, N.F.
The quantitative analysis of the pentacyclic triterpenes friedelan-3-beta-ol and friedelin by high-temperature capillary gas chromatography (HT-CGC) and high-resolution gas chromatography (HRGC) is described. These compounds are markers of Maytenus aquifolium and M. ilicifolium (Celastraceae), plants which show anti-ulcer activity and which are used medicinally in Brazil under the name "espinheira santa". HT-CGC was more efficient than conventional HRGC for the quantitative evaluation of triterpenes in the hexane extracts of leaves of M. aquifolium owing to the reduced retention times attainable without loss of resolution. The flame ionisation detector response and detection limits for both triterpenes using HRGC and HT-CGC were also established.
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