Evaluation of hypolipidemic activity of homeopathic drug Allium sativum 6C potency on different grades of dyslipidemia in Wistar albino rat models

Unhealthy dietary patterns and lifestyle modification could exacerbate heart-related diseases and the risk factors for CVD mortality includes dyslipidemia, hypertension, and diabetes mellitus. However, controlling cholesterol, triglycerides, and low-density lipoprotein could minimize the occurrence of risk factors. A homeopathic preparation of Allium sativum is effective for the treatment of various ailments, however, the usage of the drug by homeopathic practitioners for therapeutic purposes must be validated scientifically to ascertain its action. The present study is designed to evaluate the hypolipidemic and anti-inflammatory activities of the homeopathic drug A. sativum 6C in mitigating dyslipidemic changes in different types of dyslipidemic rat models. Assessment of serum biochemical parameters, evaluation of liver cytokines, and immunoblot analysis of inflammatory proteins were performed in this study. Administration of a homeopathic preparation of A. sativum 6C to different types of dyslipidemic rat model showed significant improvement in the serum lipid profile, reduction in the activity of serum marker enzymes (AST, ALT, LDH, and ALP), and also, decrement in the levels of liver inflammatory cytokines (TNFα, IL-6, and IL-1β). Further, the expression of inflammatory proteins (CRP, P-Selectin, E- Selectin) was downregulated in the liver of dyslipidemic animals upon administration of A. sativum6C. However, among the various dyslipidemic models, the efficacy of A. sativum is more profound in the HCD and HFD-fed animals. On analyzing the GC-MS data of A. sativum6C, it is shown to contain some active ingredients (Undecane; 1-methylene 1H-Indene, and Oxazolam) that might be responsible for the anti-inflammatory properties of A. sativum 6C. Homeopathic preparation of A. sativum 6Cmodulatesthe levels of serum biochemical parameters and inflammatory events in HCD-T and T2DM-T animals more than in the STZ-T animals, thus it mitigates metabolic dysfunction. Therefore, this study brings limelight to support the potential of the homeopathic drug, A. sativum for therapeutic purposes in correcting metabolic dysfunction associated with the present lifestyle.