Comparison of plant regeneration of ovules from pistils having a different number of fully-developed carpels in Thermopsis turcica
2017
Tekdal, D. | Cetiner, S.
Thermopsis turcica is a member of the Flora of Turkey and is the only representative of the genus Thermopsis in Turkey. It is commonly known as 'aci piyan, Eber sarisi, and sari meyan' among locals. Its remarkable feature is the presence of a polycarpellary gynoecium. Although it has at least 2 free carpellary ovaries, the ovular structure did not generate in each carpel Ovule development is influenced by interactions between genetic, environmental and physiological factors. The objective of the present study was to analyze whether ovules extracted from pistils having a different number of fully-developed carpels produced differences in in vitro plant regeneration. For this purpose, fruit pods consisting of 3 carpels but not all of which had generated ovules due to unexpected climatic conditions, were collected from Nezahat Gökyigit Botanical Garden of Istanbul in Turkey in 2014 for in ovule-embryo culture. The protocol used in this research consisted of dissecting embryos, culturing isolated embryos on a suitable medium, embryo development, and embryo germination to grow into a plantlet. Although in ovule-embryo culture was successful in all samples tested, callus formation was seen only on root surfaces of samples extracted from pistils having two full-developed carpels. To discover whether the calli formed were embryogenic, calli were placed on solid MS medium and were also into liquid MS medium. As a result, plantlet regeneration was observed after a month of culturing. Based on this result, we suggest that these calli can be good sources of material for cryopreservation studies on T. turcica.
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