High-Yield Production of Secreted Active Proteins by the Pseudomonas aeruginosa Type III Secretion System
2008
Derouazi, M. | Toussaint, B. | Quénée, L. | Epaulard, O. | Guillaume, M. | Marlu, R. | Polack, B.
The Escherichia coli system is the system of choice for recombinant protein production because it is possible to obtain a high protein yield in inexpensive media. The accumulation of protein in an insoluble form in inclusion bodies remains a major disadvantage. Use of the Pseudomonas aeruginosa type III secretion system can avoid this problem, allowing the production of soluble secreted proteins.
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