A fingerprint analysis method for characterization of dissolved organic matter in secondary effluents of municipal wastewater treatment plant
2014
Tang, Xin | Wu, Qian-Yuan | Zhao, Xin | Huang, Huang | Shi, Xiao-Jie | Hu, Hong-Ying
Dissolved organic matter (DOM) in wastewater and reclaimed water is related to water quality, safety, and treatability. In this study, DOM was characterized through a fingerprint analysis method for DOM characterization using resin fractionation followed by size exclusion chromatography (SEC). Resin fractionation was used in the first step to divide the DOM in water samples into six resin fractions, namely, hydrophobic acids (HOA), hydrophobic bases (HOB), hydrophobic neutrals (HON), hydrophilic acids (HIA), hydrophilic bases (HIB), and hydrophilic neutrals (HIN). SEC analysis was then performed to separate each resin fraction into several (n) subfractions with different molecular weights (MW). Thus, the total DOM in the water sample was fractionated into 6n subfractions. After quantification of each subfraction by dissolved organic carbon (DOC), a fingerprint graph was constructed to express the distribution of DOM in the subfractions. The fingerprint analysis method was applied to a secondary effluent sample during ozonation. Ozonation (dose of 10 mg L⁻¹) removed the DOC only by 8 % and reduced UV₂₅₄of the sample by 36 %. Fingerprint graphs also revealed that the resin fractions changed quite limitedly but transformation of subfractions occurred notably.
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