Quantification by fluorescent in situ hybridization of bacteria associated with Litopenaeus vannamei larvae in Mexican shrimp hatchery
2007
Garcia, A.T. | Olmos, J.S.
The bacterial composition in the hatchery at Unidad Experimental Peñasco (UEP) of the Sonora University, Mexico, was studied by using Fluorescent In Situ Hybridization (FISH) with rRNA-specific oligonucleotide probes. We applied fluorochrome-labeled polyribonucleotide probes to identify and enumerate marine shrimp culture hatchery related bacteria. Quantitative whole-cell hybridization experiments using α-, γ- and δ-Proteobacteria, and high and low G + C Gram-positive bacteria accounted for 20.8 ± 3.4% to 69.3 ± 3.3% of the total 4',6-diamidino-2-phenylindole (DAPI)-stained cells in most samples. As predicted in a previous study, marine high G + C and γ-Proteobacteria predominated in different shrimp life sub-stages. The elevated percent of high G + C and γ-Proteobacteria, extending from nauplii to mysis stages, suggest that they represent a large and significant fraction of the total picoplankton biomass in Litopenaeus vannamei larval culture.
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