Chromosome number stability and mitotic activity of cryopreserved Hypericum perforatum L. meristems
2002
Urbanová, M. | Čellárová, E. | Kimáková, K.
Meristems of in vitro-grown Hypericum perforatum L. plants were precultured for 3, 10, or 14 days in the presence of 0.5 M mannitol, or 0.076 µM or 0.76 µM abscisic acid, in RM basal liquid culture medium supplemented with 0.5 mg/l 6-benzylaminopurine and subsequently subjected to cryopreservation by the slow freezing method. The survival rate – determined as the percentage of meristems capable of differentiating plantlets – varied between 10% and 48%. Chromosome number stability of the cryopreserved meristems was determined by chromosome counting. The mitotic index of the control did not significantly differ from that of the treated samples.
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