Physiological Response to Heat Stress During Seedling and Anthesis Stage in Tomato Genotypes Differing in Heat Tolerance

Tomato cultivars differ in their sensitivity to heat stress, and the sensitivity depends on the developmental stage of the plants. It is less known how heat stress affects tomato at the anthesis stage in terms of leaf physiology and fruit set and whether the ability of tomato to tolerate heat at different developmental stages is linked. To investigate photosynthetic gas exchange characteristics, carbohydrate content and fruit set during heat stress, a thermo‐tolerant cultivar (‘LA1994’) and a thermo‐sensitive cultivar (‘Aromata’) were studied at the seedling and anthesis stage. The photosynthetic parameters, maximum quantum efficiency of photosystem II (Fᵥ/Fₘ), chlorophyll content, carbohydrate content and fruit set were determined in plants grown at 26/18 °C (control) and 36/28 °C (heat stress). The physiological responses including net photosynthetic rate (PN), chlorophyll content and Fᵥ/Fₘ decreased in ‘Aromata’ at both developmental stages during heat stress, whereas they were unaltered in ‘LA1994’ during heat stress as compared to the respective control. This was accompanied by lower contents of glucose and fructose in mature leaves of ‘Aromata’ at the seedling stage under heat stress. In contrast, the glucose content increased while the fructose content was unaltered in mature leaves of ‘LA1994’ at the seedling stage under heat stress. High temperature induced a similar change in carbohydrate content in the young leaves of both cultivars at anthesis. The fructose and sucrose content were unaffected in the mature leaves of ‘Aromata’ but significantly increased in ‘LA1994’ under heat stress at anthesis. The heat stress treatment decreased pollen viability and inhibited fruit set due to flower wilting and abnormal abscission in ‘Aromata’, whereas fruit set was not inhibited in ‘LA1994’. A decrease in chlorophyll content, photosynthesis and carbohydrate content in the mature leaves of tomato could be related to fruit set failure at high temperature. The results show that physiological responses to heat stress at the seedling stage correspond with the responses at the anthesis stage, demonstrating that screening for heat stress sensitivity can be carried out in young plants.