Purification of spinosin from Ziziphi Spinosae Semen using macroporous resins followed by preparative high‐performance liquid chromatography
2019
Li, Hongjuan | Shi, Jiling | Li, Yuanyuan | Wang, Chunhua | Hou, Guige | Cong, Wei | Zhao, Feng
As a well‐known traditional Chinese medicine, Ziziphi Spinosae Semen has been used for treating anxiety and insomnia for a long time. Spinosin, the main active C‐glycoside flavonoid in Ziziphi Spinosae Semen, has attracted much attention because of its many pharmacological activities including strong hypnotic effects, anxiolytic‐like effects, and so on. In the present work, high‐purity spinosin was separated from Ziziphi Spinosae Semen using the HPD‐300 resin followed by preparative high‐performance liquid chromatography. The adsorption kinetics curve of spinosin on the HPD‐300 resin was studied and fitted well by the pseudo‐second‐order equation. The adsorption isotherms were also constructed and low temperature favored the adsorption reaction. The separation parameters were optimized using dynamic adsorption and desorption tests. After a one‐run treatment with HPD‐300 resin, the concentration of spinosin increased 11.8‐fold from 0.99 to 11.7% with a recovery yield of 80.4%. Furthermore, the purity of spinosin could surpass above 98% after separation by preparative high‐performance liquid chromatography and recrystallization with a recovery yield of 72.6%. The developed method was effective and suitable for the large‐scale preparation of spinosin. Moreover, it was confirmed that HPD‐300 resin could enable good selection for the enrichment of flavonoids from different plants.
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