Standard system for infecting ticks (Aari: Ixodidae) with the lyme disease spirochete, Borrelia burgdorferi
1993
Piesman, J.
A standard system for infecting ticks with the Lyme disease spirochete Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwalt & Brenner is defined. Rodents infected via tick feeding or inoculation of tick homogenates were more infectious to ticks than rodents infected with culture-derived spirochetes. White laboratory mice were more infectious than hamsters. Three strains of B. burgdorferi (JD1, B31, and W1210) produced batches of infected ticks with > 80% infection when mice were infected with tick-derived material. Ixodes dammini Spielman, Clifford, Piesman & Corwin were 3.6 X more efficient than I. pacificus Cooley & Kohls in acquiring and maintaining infection with two California strains of B. burgdorferi, originally isolated from I. pacificus.
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