Novel interaction of two clock-relevant RNA-binding proteins C3 and XRN1 in Chlamydomonas reinhardtii
2012
Dathe, Hannes | Prager, Katja | Mittag, Maria
The RNA-binding protein CHLAMY1 of the green alga Chlamydomonas reinhardtii consists of two subunits, named C1 and C3 that maintain the period and phase of the circadian clock. Here, we investigated if any of its subunits interact with other clock components involved in RNA metabolism. We found that C3, but not C1 strongly interacts with exoribonuclease XRN1 whose knockout results in low amplitude rhythms. XRN1 is subject to degradation by the proteasome pathway. Its level increases in cells grown at lower ambient temperature simulating night, which was also observed for C3. Our data indicate a network of clock-relevant RNA-binding proteins. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: XRN1physically interactswithC3byanti bait coimmunoprecipitation(View interaction)XRN1physically interactswithC3bytwo hybrid(View interaction)C1physically interactswithC3bytwo hybrid(View interaction)
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