An androgen-inducible expression system for Saccharomyces cerevisiae
1991
Purvis, I.J. | Chotai, D. | Dykes, C.W. | Lubahn, D.B. | French, F.S. | Wilson, Em | Hobden, A.N.
A novel controllable expression system for Saccharomyces cerevisiae has been developed. Expression of the gene encoding the human androgen receptor, from a strong yeast promoter, results in transactivation of a hybrid promoter carrying androgen-responsive sequences such that a target gene may be expressed in an androgen-dependent manner. By selection of an appropriate combination of androgen receptor level, target-gene copy number and concentration of the androgenic ligand, dihydrotestosterone, the expression level can be set within a 1400-fold range with no detectable effect on normal cell growth.
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