Thirty-two single nucleotide polymorphism markers for high-throughput genotyping of sockeye salmon
2006
ELFSTROM, CARITA M. | SMITH, CHRISTIAN T. | SEEB, JAMES E.
We characterize 32 single nucleotide polymorphism genotyping assays for resolving genotypic variation in sockeye salmon Oncorhynchus nerka in the Pacific Rim. These assays are based on the 5'-nuclease reaction and thus facilitate high-throughput genotyping with minimal optimization time. Minor allele frequency differences (Δq) among collections were between 4.7% and 97.9%, resulting in per locus FST estimates of 0.02-0.71 with an average of 0.22.
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