Absence of Plastocyanin in the Alga Bumilleriopsis and its Replacement by Cytochrome 553
2014
Kunert, Karl-Josef | Böger, Peter
Different preparative procedures for isolation and purification of plastocyanin were successful to obtain the pure protein from Chlorella vulgaris, Scenedesm us acutus and Spinacia oleracea but not from B um illeriopsis filiform is. Lade of plastocyanin in this alga was further confirmed by the following in comparison with spinach chloroplast material: a. Use of antibodies to block photosynthetic reactions generally dependent on plastocyanin; b. epr spectral assessment; c. treatment of chloroplasts with KCN and amphotericin B ; d. light-induced changes at 553 nm (= cytochrome f photooxidation). The latter assays indicated that, in contrast to spinach, neither plastocyanin nor cytochrome f (= Cyt 553) is present in sonified or pyridine treated Bumilleriopsis chloroplasts. Photooxidation of (added) cytochrome 553 takes place not only without plastocyanin but is counteracted by the copper protein
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