NtrBC-dependent expression from the Rhizobium meliloti dctA promoter in Escherichia coli
1995
Allaway, D. | Boesten, B. | O'Gara, F.
Effects of the two-component sensor-regulator pairs DctBD and NtrBC upon the expression of a dctA::phoA fusion from Rhizobium meliloti were determined under excess and limiting nitrogen concentrations in Escherichia coli. Results indicated that NtrBC affected transcription from the detA promoter on a number of regulatory levels and under different physiological conditions in the heterologous host. However, NtrBC-dependent cross-talk was not observed in free-living R. meliloti under the conditions tested. Comparisons of the predicted amino acid sequences of DctD and NtrC from various sources indicated a specific region of the NtrC from rhizobia, which may have diverged from a consensus NtrC/DctD sequence to minimise interference between the two component systems, NtrBC and DctBD.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par National Agricultural Library
Découvrez la collection de ce fournisseur de données dans AGRIS