Pyrene Metabolism by New Species Isolated from Soil Rhizoctonia Zeae SOL3
2015
Khudhair, Ameer Badr | Hadibarata, Tony | Yusoff, Abdull Rahim Mohd | Teh, Zee Chuang | Adnan, Liyana Amalina | Kamyab, Hesam
Rhizoctonia zeae SOL3 fungus was isolated from contaminated soil based on its ability to decolorize remazol brilliant blue R in solid medium. This fungus has been used to degrade pyrene a four-ring polycyclic aromatic hydrocarbon. R. zeae SOL3 could biodegrade pyrene as a sole source of carbon and energy. Different parameters were investigated to study their effect on the biodegradation rate. The highest biodegradation rate reached at 28 °C, non-agitated culture, 20 g/L glucose, 24 g/L NaCl, and 20 mg/L pyrene. The metabolites of pyrene were detected by thin layer chromatography (TLC) and confirmed by gas chromatography–mass spectrometry (GC-MS), which were identified as benzoic acid, 4-hydroxybenzoic acid and botanic acid.
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