Glucose oxidase overproducing and negative mutants of Aspergillus niger
1990
Witteveen, C.F.B. | Vondervoort, P. van de | Swart, K. | Visser, J.
A colony staining method was used to isolate mutants in Aspergillus niger which showed altered glucose oxidase induction. The mutants were isolated under weakly or non-inducing conditions. A stable glucose-oxidase-negative mutant and a series of overproducing mutants were found. Among the overproducing mutants, different phenotypes were found with respect to glucose oxidase induction. The mutants were tested for glucose oxidase production in surface and submerged cultures, indicating a fair correspondence between those methods. From the characteristics of the mutants it can be concluded that oxygen- and carbon-source-dependent induction are mediated by different factors.
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