Quantitative glycoproteomics of high-density lipoproteins
2022
Tang, Xinyu | Wong, Maurice | Tena, Jennyfer | Zhu, Chenghao | Rhodes, C. (Christopher) | Zhou, Qingwen | Vinjamuri, Anita | Oloumi, Armin | Boddu, Sucharita | Luxardi, Guillaume | Maverakis, Emanual | Lebrilla, Carlito B. | Zivkovic, Angela M.
In this work, we developed a targeted glycoproteomic method to monitor the site-specific glycoprofiles and quantities of the most abundant HDL-associated proteins using Orbitrap LC-MS for (glyco)peptide target discovery and QqQ LC-MS for quantitative analysis. We conducted a pilot study using the workflow to determine whether HDL protein glycoprofiles are altered in healthy human participants in response to dietary glycan supplementation.
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