Chemical composition and bioactivity of water-soluble polysaccharides from leaves of Plantago notata Lagasca (Plantaginaceae) | Étude physicochimique et biologique des polysaccharides hydrosolubles de Plantago notata Lagasca (Plantaginaceae)
2015
Boual, Z. | Chouana, T. | Kemassi, A. | Hamid Oudjana, A. | Daddi Bouhoun, M. | Michaud, P. | Ould El Hadj, M. D.
Plantago notata Lagasca (Plantaginaceae), a spontaneous plant used as a traditional medicine in Ghardaïa (Septentrional Sahara Algerian). This paper reports the extraction and partial characterization of water-soluble polysaccharides from P. notata leaves. These polysaccharides were obtained by elimination of the ethanol extract and sequential extraction in distilled water, followed by precipitation in 75 % ethanol. The yield of extract is 2,0% (w/w). The crude water soluble polysaccharide extracts were further characterized and revealed the average values 08,33 ±1,05% ashes, 12,49±1,61% proteins and 77,03±3,13% carbohydrates, among them 15,66±1,06% are uronic acid and 61,35±2,13% are neutral monosaccharides. A single hydrolytic step with 4M TFA at 80°C for 4 h is suggested to be more effective in releasing monomers from polysaccharides than other hydrolysis procedures. The identification of monosaccharide composition by high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) method shows 43,95% of galactose, 20,28% of rhamnose, 11,30% of glucose 9,55% of arabinose, and 12,57% of galacturonic acid. Partial hydrolyzate of water-soluble polysaccharides was found to have a growth stimulatory effect on Lactobacillus casei less than that of fructo-oligosaccharide (RP95). However, the hydrolyzate has no effect on the Escherichia coli strain. The present study shows that partial hydrolyzate of watersoluble polysaccharides stimulates the growth of L. casei bacteria, and that partial hydrolyzate of water-soluble polysaccharides has potential use as a prebiotic health-food.
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