CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq
2016
Hashimshony, Tamar | Senderovich, Naftalie | Avital, Gal | Klochendler, Agnes | de Leeuw, Yaron | Anavy, Leon | Gennert, Dave | Li, Shuqiang | Livak, Kenneth J. | Rozenblatt-Rosen, Orit | Dor, Yuval | Regev, Aviv | Yanai, Itai
Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with threefold higher sensitivity, lower costs, and less hands-on time. We implemented CEL-Seq2 on Fluidigm’s C1 system, providing its first single-cell, on-chip barcoding method, and we detected gene expression changes accompanying the progression through the cell cycle in mouse fibroblast cells. We also compare with Smart-Seq to demonstrate CEL-Seq2’s increased sensitivity relative to other available methods. Collectively, the improvements make CEL-Seq2 uniquely suited to single-cell RNA-Seq analysis in terms of economics, resolution, and ease of use.
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