Metabolomic changes and polyunsaturated fatty acid biosynthesis during gonadal growth and development in the sea urchin Strongylocentrotus intermedius

Strongylocentrotus intermedius is an important commercial species of sea urchin distributed in the coastal waters of China. However, the metabolomic changes that accompany its gonadal growth and development stages remain unclear. In this study, we have histologically observed gonad growth stages, analyzed the fatty acid composition, and employed an untargeted metabolomic approach to investigate the metabolites associated with the gonadal growth and development of S. intermedius, as well as the biosynthesis and metabolism of polyunsaturated fatty acids (PUFAs) at different stages and in different sexes. The gonad mass of sea urchin increased from 0.70 ± 0.18 g in January (at the recovering stage) to 8.78 ± 2.89 g in July (the reproductive stage), with the GSI increasing from 4.02 ± 0.88% to 16.86 ± 2.79%. We have analyzed 34 types of fatty acids, such as arachidonic acid, eicosapentaenoic acid, etc., of which PUFAs were the dominant fatty acid class in this species, accounting for >48.55% of the total. In the metabolomic analysis, linolelaidic acid, sciadonic acid, cis-8,11,14,17-eicosatetraenoic acid, adrenic acid, docosapentaenoic acid, and tetracosapentaenoic acid were detected in the differentially expressed metabolites of the unsaturated fatty acids biosynthesis pathway. We found that the most significant functional pathways during gonadal growth and development were “arachidonic acid metabolism”, “alpha-linolenic acid metabolism” and “linoleic acid metabolism”, which are all related to fatty acid metabolism. These results will provide valuable information on the possible presence of both exogenous and endogenous fatty acids in sea urchin gonads and the metabolomic changes in S. intermedius during gonadal growth periods, and will further our understanding of the intermediary metabolism and the molecular bases of growth traits in this species.