Production of aflatoxin in corn by a large-scale solid-substrate fermentation process
1979
Silman, R.W. | Conway, H.F. | Anderson, R.A. | Bagley, E.B.
Culturing of Aspergillus flavus was conducted in static flask cultures and 4 in. .times. 5 ft columns (containing 7-8 kg corn) to measure the effects of moisture, temperature and air flow on growth and the production of aflatoxin. Aflatoxin levels as high as 6200 ppb (dry basis) in 10 days were observed. Conditions were selected (about 20% moisture, 0.008 l air/kg corn per min air flow with 1.5 l/kg per min recirculated) for production of aflatoxin in 1200 bushels of corn in a 18 ft diameter corrugated steel Butler storage bin for preparation of contaminated corn for animal feeding trials and for testing of an ammoniation process for decontamination of aflatoxin in corn. A target level of 100 ppb aflatoxin was attained.
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