Cytotoxic effects of bioactive extracts from Andrographis echioides (L.) Nees: An In vitro approach
2022
Devanesan, Sandhanasamy | Ranjitsingh, Amirtham J. | AlSalhi, Mohamad S. | Murugan, A. | Abd Elkader, Omar H. | Nicoletti, Marcello | Brindhadevi, Kathirvel
In the global search for alternative drugs for various ailments, products from medicinal plants are given much attention. Attempts are being made to identify bioactive compounds from natural products to develop anticancer agents. In the present study exposes the cytotoxic activity of the extracts of the leaves of the plant Andrographis echioides on cancer cell lines. A. echioides leaf extract was taken with polar grade solvents, and methanol was selected based on the best activity. The fractions, obtained by column chromatography separation, rich in bioactive compounds were further screened for anticancer studies, using cancer cell lines, MCF-7, A- 549, and normal Vero cells. Cell proliferation assay, Western blotting, flow cytometry, DNA fingerprinting, and apoptosis assessment were used to identify the anticancer potential of the chosen methanol extract fractions of A. echioides. The IC50 values of the 3′-O-β-D-xylosyl-quercetin (AEMR-1) isolated from A. echioides were 1.25, 1.98 µg/mL, and 4.54 µg/mL for the MCF-7, A-549, and Vero cell lines, respectively. The normal Vero cell line remained unchanged on AEMR-1 exposure. The study's outcome will help to find out the lead compounds in the plant A. echioides useful for chemotherapy in cancer treatment.
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