Large-scale in vitro propagation of Impatiens repens Moon., a critically endangered medicinal plant in Sri Lanka
2010
Herath, H.M.I. | Wijesundara, D.S.A.
Impatiens repens Moon. (Ceylon Balsam: Family Balsaminaceae) is an endemic, critically endangered, medicinal plant in Sri Lanka. Habitat destruction and over-exploitation have resulted in the virtual extinction of this species. To grow this plant sustainably, a micropropagation protocol was developed using nodal explants for rapid, large-scale propagation. Nodal segments were established on Murashige and Skoog (MS) medium supplemented with different combinations of 6-benzylaminopurine (BAP) and Kinetin for shoot induction. Shoots were established on MS medium supplemented with different combinations of BAP and indole-3-acetic acid (IAA) for shoot multiplication. Micro-shoots were established on full-strength or half-strength MS medium supplemented with different concentrations of α-naphthalene-acetic acid (NAA) for root induction. MS medium supplemented with 2.0 mg l–¹ BAP and 1.0 mg l–¹ Kinetin gave the highest mean number of shoots per nodal explant (4.7), the highest mean shoot length (3.2 cm), and the highest frequency of shoot regeneration (100%). Addition of a combination of BAP and IAA significantly influenced shoot multiplication. The highest mean number of shoots per shoot explant (18.8) was observed on MS basal medium supplemented with 2.0 mg l–¹ BAP and 0.1 mg l–¹ IAA. The highest mean number of roots per shoot, mean root length, and frequency of rooting were observed on half-strength MS medium supplemented with 1.0 mg l–¹ NAA. Plantlets were acclimatised, with a 96% survival rate, on a 3:1 (v/v) mix of sand: compost (leaf mould).
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