The enzymes and electrolytes profiles in hydatid cyst fluid of naturally infected Iranian domestic ruminants

Cystic echinoccoccosis is a zoonotic and prevalent disease which causes health problems and economic looses worldwide. The biochemical components of hydatid cyst fluid (HCF) have important role in metabolism and growth of unilocular hydatid cyst. The aim of the current study was to determine HCF level of enzymes and electrolytes profiles in naturally infected Iranian domestic ruminants. The livers and lungs infected with hydatid cysts were collected and HCF was aspirated from a total of 100 cysts obtained from livers (50 cysts) and lungs (50 cysts) of slaughtered domestic ruminants. Biochemical measurement of HCF was undertaken using colorimetric technique, refractometer, flame photometer, and biuret method. The enzyme levels of HCF were also measured by using appropriate kits. The average HCF level of calcium (Ca) was found to be significant higher in cattle (7.49 ± 1.28 mg/dl) and goats (13.98 ± 5.11 mg/dl) lungs. The average HCF level of phosphorous (P) was significantly higher in cattle livers (0.82 ± 0.02 mg/dl) than other examined animals. Measurement of the average HCF level of magnesium (Mg) was significantly lower in camel lungs (11.8 ± 1.05 mg/dl) than that in cattle livers (10.56 ± 1.73 mg/dl). The highest average HCF levels of Natrium (Na, 122.8 ± 11.91 mEq/l) and Kalium (Ka, 7.18 ± 1.37 mEq/l) were measured in sheep livers. The average HCF level of albumin (Alb) in infected lungs was significantly lower in cattle (0.48 ± 0.01 mg/ml) than camels (0.95 ± 0.05 mg/ml). The average HCF level of total protein (TP) in infected lungs was also lower in sheep (0.51 ± 0.06 mg/dl) than goats (3.21 ± 0.51 mg/dl). The highest average HCF level of creatine phosphokinase (CPK, 1229.25 ± 13.21 U/ml) and lactate dehydrogenase (LDH, 363.62 ± 10.44 U/ml) were measured in infected lungs of camels. It was concluded that HCF level of enzymes and electrolytes had differences in examined Iranian domestic ruminants which may be used to screen and help in characterization and identification of strains of Echinococcus granulosus.