Evaluation of the antioxidant capacity, volatile composition and phenolic content of hybrid Vitis vinifera L. varieties sweet sapphire and sweet surprise
2022
de Freitas Laiber Pascoal, Gabriela | de Almeida Sousa Cruz, Marta Angela | Pimentel de Abreu, Joel | Santos, Millena Cristina Barros | Bernardes Fanaro, Gustavo | Júnior, Mário Roberto Maróstica | Freitas Silva, Otniel | Moreira, Ricardo Felipe Alves | Cameron, Luiz Claudio | Simões Larraz Ferreira, Mariana | Teodoro, Anderson Junger
Bioactive compounds were extracted using two different extraction solvents (acetone and water) from pulp and whole grape berries derived from hybrid Vitis vinifera L. varieties Sweet sapphire (SP) and Sweet surprise (SU) and were characterised based on a comprehensive metabolomic approach by chromatography coupled with mass spectrometry (UPLC-QTOF-MSE and GC-FID/MS). GC-FID/MS analysis was performed with two different extraction methods (solvent extraction method and solid-phase extraction). Anthocyanins were characterised and quantified by HPLC-UV. The antioxidant potential was assessed by different assays. SP acetone extract from grape skin had the highest mean to DPPH, FRAP, ORAC and phenolic content SP samples, also showed higher anthocyanin content. Globally, 87 phenolic compounds were identified. The relative quantification by UPLC-MSᴱ showed flavonoids the most abundant class. Forty two compounds were found in the volatile fraction of SU, while only thirty one volatile compounds were found in the SP samples.
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