Distribution of glycinin in the gastrointestinal tissue of pigs at different growth stages
2013
Zhao, Yuan | Zhang, Bing | Qin, Guixin | Wang, Tao | Wang, Jun | Han, Rui
The gastrointestinal distribution of glycinin in pigs at different growth stages was investigated in this study. Fifteen healthy General No. 1 barrows weaned on the 28th day were randomly assigned to three groups with five replicates: five weanling, five growing and five finishing. All pigs received diets with non-soya bean ingredients in non-experimental periods, while the pigs received diets containing 4% purified glycinin in experimental periods. Binding of anti-glycinin antibody was detected using a labelled streptavidin–peroxidase complex method to determine the gastrointestinal distribution of glycinin. The results indicated that there was a significant difference on the gastrointestinal mucosal distribution of glycinin between pigs of different ages. Glycinin in growers was higher than piglets and lower than finishers (P <0.001). The highest content of glycinin was in the distal jejunum and ileum for piglets, and in distal jejunum for growers and finishers (P <0.05).
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