Automated system to quantify the behavior of small insects in a four-pointed star olfactometer
1997
Vigneault, C. | Panneton, B. | Cormier, D. | Boivin, G.
The construction, assembly and testing of an improved four-pointed star olfactometer are described. Modifications to earlier versions were motivated by the need to automate collection and analysis of data on insect locomotive response to airborne stimuli. A machine vision system capable of resolving small insects (<1 mm) in arenas of surface area 100,000 times greater than the surface area of the insect was assembled from commercially-available components. A real-time insect positioning and tracking algorithm was developed. A software package for data quality control and computation of insect movement parameters completed the system. Trials conducted with the parasitoid Anaphes listronoti (Hymenoptera: Mymaridae) (dimensions 200 by 700 micrometer) demonstrate that the system provides accurate data on insect locomotive response.
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