Isolation and Identification of Polymethoxyflavones from the Hybrid Citrus, Hallabong
2010
Han, Saem | Kim, Hye Min | Lee, Jeong Min | Mok, So-Youn | Lee, Sanghyun
Seven polymethoxyflavones (PMFs) were isolated from the dried peels of hallabong, the hybrid Citrus, by a repeated column chromatography. The structures of PMFs were identified as 5,6,7,3′,4′-pentamethoxyflavone (1), 6,7,8,3′,4′-pentamethoxyflavone (2), 3-hydroxy-5,6,7,4′-tetramethoxyflavone (3), 5,6,7,8,3′,4′-hexamethoxyflavone (4), 3,6,7,4′-tetramethoxyflavone (5), 3,5,6,7,8,3′,4′-heptamethoxyflavone (6), and 5,6,7,8,4′-pentamethoxyflavone (7) using 1H and 13C NMR in combination with mass spectrometry. Among these compounds, 5 was isolated for the first time from nature. The content of PMFs 1−7 in hallabong was determined by HPLC-UV. The major PMFs of hallabong are 5 in the dried peels (15.4 mg/g) and 7 in the dried leaves (12.2 mg/g).
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