Modulation of immune genes mRNA levels in mucosal tissues and DNA damage in red blood cells of Sparus aurata by gold nanoparticles
2018
Teles, M. | Reyes-López, F.E. | Fierro-Castro, C. | Tort, L. | Soares, A.M.V.M. | Oliveira, M.
Gold nanoparticles (AuNP) effects on Sparus aurata were evaluated on skin, gills and intestine by assessing the expression of immune genes and in peripheral blood evaluating genetic damage. Fish were exposed to 0.5 and 50 μg/L AuNP for 96 h. Results showed that exposure to 50 μg/L AuNP induced an upregulation in the expression of innate immune genes in gills (c3, lys, il1β, tnfα, il6, il10 and tgfβ) and intestine (il1β, tnfα and il6). Furthermore, mRNA levels of hsp70 and hsp90 were increased in gills after exposure to 0.5 μg/L AuNP, when compared to 50 μg/L. Present data demonstrated the sensitivity of gills and intestines to AuNP exposure supporting their use in the study of fish responses to other nanoparticles. Genotoxic potential of AuNP was demonstrated by increased DNA strand breaks in red blood cells of fish exposed to AuNP, suggesting that AuNP represent a potential hazard to fish.
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