Hyaluronic acid as a substitute for proteins in the deep-freezing of embryos from mice and sheep: an in vitro investigation
1992
Joly, T. | Nibart, M. | Thibier, M.
The aim of the present study was to investigate the ability of frozen-thawed mouse and sheep embryos to develop in vitro after introducing hyaluronic acid (HA) into the freezing medium as a substitute for biological proteins. A total of 443 mouse embryos and 120 sheep embryos were divided into equal numbers to be frozen in one of two freezing media containing either 4 mg/ml BSA (control) or 1 mg/ml HA (treated). Overall, 80% of the mouse embryos developed after thawing, with no significant difference (P > 0.05) between the two freezing media. Similarly, 75% of the frozen-thawed sheep embryos developed in culture after thawing, with no differences (P > 0.05) between the two groups. It was concluded that although the handling of embryos is more difficult with the HA compound than with BSA, the HA compound may be safely substituted for BSA for international movement of embryos, if these preliminary results are confirmed in vivo.
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