Total Biosynthesis of Brassicicenes: Identification of a Key Enzyme for Skeletal Diversification
2018
Tazawa, Akihiro | Ye, Ying | Ozaki, Taro | Liu, Chengwei | Ogasawara, Yasushi | Dairi, Tohru | Higuchi, Yusuke | Kato, Nobuo | Gomi, Katsuya | Minami, Atsushi | Oikawa, Hideaki
The biosynthetic pathway of brassicicenes, derived from the phytopathogen Pseudocercospora fijiensis, was fully reconstituted. Heterologous expression of the eight genes highly expressed in infected leaf tissues generated a new brassicicene derivative as a final product. Together with the characterization of P450 from Alternaria brassicicola, a late stage of the biosynthetic pathway, which generates remarkable structural diversity, has been proposed. Notably, a unique P450 that converts 3 to the structurally distinct 4 and 6 was identified.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par National Agricultural Library
Découvrez la collection de ce fournisseur de données dans AGRIS