Syntheiss of aromatic n-alkyl-glucoside esters in a coupled beta-glucosidase and lipase reaction
1998
Otto, R.T. | Bornscheuer, U.T. | Syldatk, C. | Schmid, R.D.
Regioselective synthesis of e.g. 6-O-phenylbutyryl-1 -n-butyl-beta-D-glucopyranose was achieved in 21% yield using almond-beta-glucosidase and Candida antarctica lipase B. The beta-glucosidase reaction was performed in a biphasic (buffer/n-alcohol) system using free and Eupergit C-immobilized glucosidase. Immobilized enzyme allowed product formation even at a water content of 1%.
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