Cytotoxic response of platinum‐coated gold nanorods in human breast cancer cells at very low exposure levels
2016
Ahamed, Maqusood | Akhtar, Mohd Javed | Khan, M. A Majeed | Alhadlaq, Hisham A. | Alrokayan, Salman A.
Because of unique optical behavior gold nanorods (GNRs) have attracted attention for the application in biomedical field such as bio‐sensing, bio‐imaging and hyperthermia. However, toxicological response of GNRs is controversial due to their different surface coating. Therefore, a comprehensive knowledge about toxicological profile of GNRs is necessary before their biomedical applications. First time, we investigated the toxic response of GNRs coated with platinum (GNRs‐Pt) in human breast carcinoma (MCF‐7) cells. Platinum coating further improves the optical and catalytic properties of GNRs. Assays such as 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazoliumbromide (MTT), neutral red uptake (NRU) and lactate dehydroganase (LDH) assays have shown that GNRs‐Pt induced cytotoxicity at very low exposure levels (0.1–0.8 μg mL⁻¹). Accumulation of cells in SubG1 phase and low mitochondrial membrane potential (JC‐1 probe) in treated cells suggest that GNRs‐Pt induced cell death via apoptotic pathway. Quantitative real‐time PCR data demonstrated that mRNA expression of apoptotic genes (bax, caspase‐3 and caspase‐9) were up‐regulated while anti‐apoptotic gene bcl‐2 was down‐regulated in cells exposed to GNRs‐Pt. We further observed the higher activity of caspase‐3 and caspase‐9 enzymes in GNRs‐Pt treated cells supporting mRNA data. Moreover, N‐acetyl cysteine (NAC) significantly attenuated the ROS generation and cytotoxicity induced by GNRs‐Pt in MCF‐7 cells suggesting that ROS might plays a crucial role in GNRs‐Pt induced toxicity. This study warns of possible toxicity of GNRs even at very low exposure levels. Further investigations needed to explore potential mechanisms of this low dose toxicity phenomenon. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1344–1356, 2016.
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