Determination of androstenone in pig fat using supercritical fluid extraction and gas chromatography-mass spectrometry
1995
Magard, M.A. | Berg, H.E.B.
A method for the analysis of androstenone (5 alpha-androst-16-en-3-one) in boar fat samples is described. Sample workup is based on supercritical fluid extraction (SFE), with final determination using gas chromatography-mass spectroscopy (GC-MS) in selected ion monitoring (SIM) mode. Careful tuning of the supercritical fluid density and extraction temperature enables fairly selective extraction of the target analyte. Further selectivity can be obtained by choosing alumina as the support on which the fat is loaded, as indicated by full-scan chromatograms. The extraction can be speeded up by effective spreading of the sample on the support and by using the lowest possible amount of sample with acceptable precision and accuracy in the quantitation. The detection limit for androstenone was 0.05 micrograms/g of fat using optimized conditions. No significant differences in accuracy were found when the SFE/GC-MS method was compared with two other methods (based on radioimmunoassay and liquid chromatography) designed for the analysis of androstenone in pig fat.
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