Nutritional impact of low-cost substrates on biphasic fermentation for conidia production of the fungal biopesticide Metarhizium anisopliae
2021
Santos, Polyane de Sá | Abati, Kauana | Mendoza, Natalie Verónika Rondinel | Mascarin, Gabriel Moura | Delalibera Júnior, Italo
Agro-industrial wastes (by-products) were used in a biphasic fermentation system for the optimal production of Metarhizium anisopliae conidia. Submerged fermentation carried out with 45 g L⁻¹ glucose and 36 g L⁻¹ yeast extract rendered the highest blastospores yield within four days. Blastospores were used as the inoculum source in solid-state fermentation alternatively to conidia suspension. The solid substrates included rice, bran, and inert supports for growth and sporulation. Pre-cooked rice inoculated with blastospores showed higher yield and faster conidia production compared to inoculation with conidia. The optimal conidia production was obtained after seven days when inoculated with blastospore-based pre-culture. Conidia production after fungal growth on wheat bran (1.2 × 10⁹ conidia g⁻¹) was twice as high as on corn bran. Sugarcane bagasse was the best supporting matrix for growth and sporulation (5.14 × 10⁸ conidia g⁻¹). This study highlights the feasibility of a cost-effective biphasic liquid-solid fermentation of M. anisopliae.
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