Geographical Differentiation of Hom Mali Rice Cultivated in Different Regions of Thailand Using FTIR-ATR and NIR Spectroscopy
2021
Srinuttrakul, Wannee | Mihailova, Alina | Islam, Marivil D. | Liebisch, Beatrix | Maxwell, Florence | Kelly, Simon D. | Cannavan, Andrew
Although Hom Mali rice is considered the highest quality rice in Thailand, it is susceptible to adulteration and substitution. There is a need for rapid, low-cost and efficient analytical techniques for monitoring the authenticity and geographical origin of Thai Hom Mali rice. In this study, two infrared spectroscopy techniques, Fourier-transform infrared spectroscopy with attenuated total reflection (FTIR-ATR) and near-infrared (NIR) spectroscopy, were applied and compared for the differentiation of Thai Hom Mali rice from two geographical regions over two production years. The Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) model, built using spectral data from the benchtop FTIR-ATR, achieved 96.97% and 100% correct classification of the test dataset for each of the production years, respectively. The OPLS-DA model, built using spectral data from the portable handheld NIR, achieved 84.85% and 86.96% correct classification of the test dataset for each of the production years, respectively. Direct NIR analysis of the polished rice grains (i.e., no sample preparation) was determined as reliable for analysis of ground rice samples. FTIR-ATR and NIR spectroscopic analysis both have significant potential as screening tools for the rapid detection of fraud issues related to the geographical origin of Thai Hom Mali rice.
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