Intracellular localization of glutelin mRNA during grain development in rice
1990
Ramachandran, C. | Raghavan, V.
Glutelin is the major storage protein of rice, accounting for about 80% of the total protein in the starchy endosperm. We have employed an in situ hybridization technique using RNA probes to monitor the spatial and temporal expression of the glutelin gene during the development of rice grain. Annealing of sections of the grain with 35S-labelled antisense transcripts showed that gene expression is switched on between 4 DA (day after anthesis) and 6 DA. Although glutelin mRNA was transcribed in the pericarp, endosperm and embryo of the developing grain, the time of its peak accumulation varied in the different tissues. A steady increase in the concentration of glutelin mRNA was observed in the pericarp and endosperm cells from 6 DA to 18 DA. This was followed by a progressive reduction and complete disappearance of transcripts by 24 DA. Glutelin transcripts were maximally present in the embryo about 8 DA. Northern blot and dot blot hybridization of 32P-labelled glutelin gene to rice grain poly(A) + RNA and total RNA, respectively, confirmed the temporal pattern of gene expression revealed by in situ hybridization.
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