Surfactant-enhanced anaerobic acidogenesis of Canna indica L. by rumen cultures
2008
Yue, Zheng-Bo | Yu, Han-Qing | Hu, Zhen-Hu | Harada, Hideki | Li, Yu-You
Polyoxyethylene sorbitan monoolate (Tween 80) was used to enhance the anaerobic acidogenesis of Canna indica L. (canna) by rumen culture in this study. Dose of Tween 80 at 1ml/l enhanced the volatile fatty acids (VFA) production from the acidogenesis of canna compared to the control. However, Tween 80 at higher dosages than 5ml/l inhibited the rumen microbial activity and reduced the VFA yield. Response surface methodology was successfully used to optimize the VFA yield. A maximum of VFA yield of 0.147g/g total solids (TS) added was obtained at canna and Tween 80 concentrations of 6.3g TS/l and 2.0ml/l, respectively. Dosage of Tween 80 at 1-3.75ml/l reduced the unproductive adsorption of microbes or enzymes on the lignin part in canna and increased microbial activity. A high VFA production was achieved from canna presoaked with Tween 80, suggesting that the structure of canna was disrupted by Tween 80.
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