Effects of Growth Conditions and Cultivar on the Content and Physiochemical Properties of Arabinoxylan in Barley
2020
Marconi, Ombretta | Tomasi, Ivan | Sileoni, Valeria | Bonciarelli, Umberto | Guiducci, M. (Marcello) | Maranghi, Stefano | Perretti, Giuseppe
The present study aims to evaluate the effect of the growth conditions and the cultivar on the total and water-extractable (W-E) arabinoxylan (AX) in barley. For this purpose, nine barley varieties from two different years were analyzed. The total AX content ranged from 5.97 to 8.98 wt % d.m., while the W-E AX ranged from 0.06 to 0.35 wt % d.m. The W-E AX molecular properties were characterized by high-pressure size exclusion chromatography (HPSEC)-triple detector array (TDA). The molecular weight was between 2.3 × 10⁵ and 12.6 × 10⁵ Da, the polydispersity was between moderate and broad (1.1 < Mw/Mₙ < 4.3), and the conformation was a stiff semiflexible coil (0.5 < α < 1.3). The results indicate that the year influences the content of total AX and W-E AX and some molecular characteristics of W-E AX, such as its polydispersity and its conformation. Finally, the results demonstrated that the W-E AX can be used as an index of the malting attitude of barley because it positively correlates with germinative energy and kernel dimension.
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