Yields of alginates produced by fluorescent pseudomonads in batch culture
1995
Fett, W.F. | Wijey, C.
Saprophytic and plant pathogenic fluorescent pseudomonads are possible sources of bacterial alginates to be used as substitutes for algal alginates for certain commercial applications. In this study, a total of 115 strains of fluorescent Pseudomonas species (P. cichorii, P. fluorescens, P. syringae and P. viridiflava) were tested for yields of alginates when grown in batch culture in a proprietary liquid medium (PLM). The PLM contained either fructose or glucose (both at 5%, w/v) as the primary carbon and energy source. For comparison, selected strains were also grown in a modified Vogel and Bonner medium (MVBM) containing gluconate (5%, w/v) and formulated to support maximal alginate production by the human pathogen P. aeruginosa. After five days of incubation at 24 degrees C with shaking (250-300 r.p.m.), alginates were harvested from the culture fluids by precipitation with three volumes of isopropanol. Maximum yields of alginates, based on assays for uronic acid content of precipitable material, were 5 g L(-1) for PLM with fructose, 3 g L(-1) for PLM with glucose and 9 g L(-1) for MVBM.
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