Mathematical modeling of poly[(R)-3-hydroxyalkanoate] synthesis by Cupriavidus necator DSM 545 on substrates stemming from biodiesel production
2013
Vrana Špoljarić, Ivna | Lopar, Markan | Koller, Martin. | Muhr, Alexander | Salerno, Anna | Reiterer, Angelika | Malli, Karin | Angerer, Hannes | Strohmeier, Katharina | Schober, Sigurd | Mittelbach, Martin | Horvat, Predrag
Two low structured mathematical models for fed-batch production of polyhydroxybutyrate and poly[hydroxybutyrate-co-hydroxyvalerate] by Cupriavidus necator DSM 545 on renewable substrates (glycerol and fatty acid methyl esters-FAME) combined with glucose and valeric acid, were established. The models were used for development/optimization of feeding strategies of carbon and nitrogen sources concerning PHA content and polymer/copolymer composition. Glycerol/glucose fermentation featured a max. specific growth rate of 0.171h−1, a max. specific production rate of 0.038h−1 and a PHB content of 64.5%, whereas the FAME/valeric acid fermentation resulted in a max. specific growth rate of 0.046h−1, a max. specific production rate of 0.07h−1 and 63.6% PHBV content with 4.3% of 3-hydroxyvalerate (3HV) in PHBV. A strong inhibition of glycerol consumption by glucose was confirmed (inhibition constant ki,G=4.28×10−4gL−1). Applied concentration of FAME (10–12gL−1) positively influenced on PHBV synthesis. HV/PHBV ratio depends on applied VA concentration.
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