Prevalence and Characteristics of Quinolone Resistance in Salmonella Isolated from Retail Foods in Lanzhou, China
2019
Chen, Qiwei | Gong, Xiongwei | Zheng, Fuying | Ji, Peng | Yuan, Ziwen | Liu, Yongsheng | Wei, Yanming
The aim of this study was to determinate the prevalence of Salmonella in retail foods and its resistance to quinolones in retail foods in Lanzhou, People's Republic of China. In this work, 2,182 food samples, collected from March 2015 to December 2018, were analyzed to detect Salmonella and then analyzed for serotype distribution, quinolone resistance, and quinolone-resistant gene detection. The findings demonstrate that the overall prevalence of Salmonella in these food categories was low. A total of 41 (1.9%) of 2,182 food samples were found to be positive for Salmonella. Ten distinct serovars were identified, and Salmonella Derby, Salmonella Anatum, and Salmonella Enteritidis were the most prevalent serovars. According to the broth microdilution test, the resistance percentages were 90.2% to nalidixic acid, 39.0% to enrofloxacin, 41.5% to ciprofloxacin, 29.3% to ofloxacin, and 26.8% to levofloxacin. Among the quinolone-resistant isolates, 12 strains had a single mutation in gyrA at codon 83 (Ser→Phe) or codon 87 (Asp→Asn or Asp→Gly). Five isolates had one parC mutation (Ser80→Arg) and one or two gyrA hot spot mutations. qnr genes were found in seven isolates (five qnrB and two qnrD), and the aac(6′)-Ib gene in seven isolates. Two isolates carry both qnrB and aac(6′)-Ib-cr genes. Based on these results, a low prevalence of Salmonella contamination in retail foods was found, but it might play a potential risk factor in the spread of quinolone-resistant Salmonella strains in the Lanzhou region.
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