Purification of the mitochondrial citrate transporter in yeast

Persson, L.O.; Srere, P.A.

Purification of the mitochondrial citrate transporter in yeast

1992

Persson, L.O. | Srere, P.A.

We present a method of partial purification of mitochondrial citrate transporter of the yeast Saccharomyces cerevisiae. Based on functional evidence of interaction between citrate transport and citrate synthase, we have used an affinity column containing pig heart citrate synthase (PHCS) for the purification. The purified preparation shows two protein components whose Mr is 50K and 60K. The specific activity of our purest fractions is 2.6 micromoles/min which compares favorably to that of purified beef liver enzyme and purified rat liver enzyme.

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Mots clés AGROVOC

carrier proteins chemistry chromatography citric acid enzymology immunology lyases microbial proteins mitochondria mitochondria purification saccharomyces cerevisiae

Informations bibliographiques

Publié dans

Biochemical and biophysical research communications

Volume 183 Numéro 1 Pagination 70 - 76 ISSN 0006-291X

D'autres materias

Affinity; Isolation & purification; Citrate (si)-synthase; Citrate carrier; Carriers; Liposomes

Langue

anglais

Note

2019-12-04

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-28

Format: MODS

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Purification of the mitochondrial citrate transporter in yeast

1992

Mots clés AGROVOC

Informations bibliographiques