Potential Utilization of Dagaa (Rastrineobola argentea) Protein Hydolysate in Formulation of Media to Support Microbial Growth and Production of Bacterial Secondary Metabolites
2021
Pere, Katherine | Muge, Edward K. | Mbatia, Betty N. | Wekesa, Vitalis W.
Dagaa fish hydrolysate (DFH) and dagaa protein hydrolysate (DPH) were prepared from dagaa using Alcalase enzyme. Proximate, mineral analysis, and potential use of DFH and DPH in microbial media were evaluated. Dagaa media formulations were used to culture bacteria commonly used for amylase production (Bacillus subtilis and Rhodobacter capsulatus) and that used as a bio-pesticide (Metarhizium anisopliae). Whole dagaa had the highest protein (5.2 ± 0.1 mg/ml), ash (13.5 ± 0.4%) and fat (2.36 ± 0.2%) content, while DPH had the least protein (2.6 ± 0.0 mg/ml) content. Mineral content was significantly different (p < 0.05) between dagaa and its hydrolysates. Dagaa and LB had the highest Sodium (22.7 ± 1.1 mg/ml) and calcium (0.3 ± 0.0 mg/ml), content respectively. Sustenance of amylase production in DPH-starch and standard media by both microorganisms was significantly different (p < 0.05). Sporulation rate of M. anisopliae in experimental media was not significant (p ˃ 0.05).
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